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2019年7月12日 · An exostosis, also called an osteoma, is a benign overgrowth of a pre-existing bone. It forms during the active growth stage. In the majority of cases the bone overgrowth is capped with cartilage,...
- Introduction
- Fixation
- Embedding
- Sectioning
- Mounting
- Staining
- Coverslipping
- Summary
- References
- About Huron Digital Pathology
Histology, the study of the structure of cells and their formation into tissues and organs, forms the basis of much of our understanding of cell function and disease processes. Histotechnology allows the structure and organization of tissues to be visualized. In addition to facilitating the study of cell biology, this is a powerful tool in the diag...
Fixation is required to preserve the tissue sample in a state as close to that during life as possible. Fixation should thus be carried out as soon as possible after removal of the tissues. A range of fixatives are available and, although none is perfect, formaldehyde is generally considered to be the fixative of choice. Formalin is also known to p...
The preserved tissue is now ready to be embedded in a medium to allow thin slices to be cut cleanly. The medium most commonly used is wax, such as paraffin. Paraffin melts at about 56–58°C and so is safe for embedding neurological tissue. To prevent the tissue becoming soggy, which will make sectioning difficult, all the water must be removed from ...
Once cooled, the fixed and processed tissue blocks can be sectioned. When using a microtome to produce large sections, static electricity can be a real problem. Operating a small humidifier close to the sliding microtome and setting up in an area where there are few air currents can help to minimize these unwanted effects. “A good trick is to have ...
Prior to mounting, the tissue sections should be floated on a warm water bath and the glass slides warmed in an oven. The heating of the section helps ensure that the tissue is smooth and the increased flexibility makes it easier to transfer to the slide. Larger sections are more prone to collapsing and so should be picked up on the glass slide car...
Dyes are used in histology to differentiate features of interest. A range of dyes of differing composition and reactions are available. Knowledge of the properties of a particular dye make it possible to distinguish various tissue structures by dying them different colors. Unfortunately, dyes are water soluble and so will not stain tissues containi...
The stained section on the slide must then be protected with a coverslip to protect the tissue from being scratched and to preserve the tissue section. It is then necessary to once again remove all the water from the section by subjecting it to the reverse of the process used to remove the paraffin for staining. The stained slide should be passed t...
Imaging of histology samples has been fundamental to our understanding of how the various systems in the body function and how they malfunction in different disorders. The value of these images depends on the precise preparation of tissue sections in readiness for scanning. The tissue must be expertly preserved and processed in order to obtain the ...
Hua Y, et al. Nature Communications 2015; volume 6 article 7923. Available at http://www.nature.com/articles/ncomms8923KapelsohnK. Protocol Exchange 2015.Published online 17 March 2015. Available at http://www.nature.com/protocolexchange/protocols/3745#/procedurePinskiy V. et al. PLOS Published online 16 July 2015. Available at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102363Based in Waterloo, Ontario, Canada, Huron Digital Pathology has a 20 year history designing sophisticated imaging instrumentation. Our end-to-end digital whole slide imaging solutions for digital pathology incorporate our award-winning TissueScope™ digital slide scanners; TissueView™ image viewing, sharing and management platform; and our workflow-...
2022年12月9日 · Destruction or loss of small nerve fibers results in pain, tingling, numbness or a burning sensation in the lower extremities, with a distal to proximal gradient. Affected individuals...
2021年3月31日 · By the third month after the baby’s birth the soft spot on the rear of the skull is usually sealed up as the suture knits the bones together. As the suture solidifies, the back of the skull is...
2023年2月20日 · Noma, also known as cancrum oris or gangrenous stomatitis, is a severe and aggressive gangrenous process (a condition wherein body tissues die due to infection or lack of blood supply) that affects...
2019年2月26日 · Advanced clubbing - hypertrophic osteoarthropathy - involves a painful growth of connective tissue in long bones such as the tibia or radial bone. This inflammation occurs with a fever and joint...
2019年4月1日 · Iridoschisis is a condition described by Schmitt in 1922, but so named in another case report by Lowenstein and Foster in 1945. In this condition, the front portion of the iris (anterior layer of...
