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搜尋結果
2021年1月4日 · Although studies had different designs, e.g. not using random allocation and/or blinding, our findings suggest that maternal use of some dietary supplements, including vitamin A, D, vitamin B1, B2 and vitamin C might be reflected in human milk. Vitamin supplements had agreater effect on breast milk composition compared to minerals.
- Mojtaba Keikha, Ramin Shayan-Moghadam, Maryam Bahreynian, Roya Kelishadi
- 2021
- Study Setting
- Demographic and Anthropometric Variables Evaluation
- Milk Sampling
- Oxidant-Antioxidant Status Measurement
- Statistical Analysis
This cross-sectional study was performed at Birjand University of Medical Sciences on a sample of 350 mothers who were recruited between January–February 2021 from 4 health centers in different regions of Birjand, South Khorasan, Iran. Inclusion criteria included; healthy women between the ages of 20–35 years, with an infant 1–6 months old. We did ...
A trained nurse evaluated information including mother age, systolic blood pressure (SBP), type of delivery, body mass index (BMI), economic status, parent death, parent divorce, father s’ and mother s’ educational attainment, infant age, infant sex and infant head circumference. Participants’ height and weight were measured and BMI was calculated ...
Human milk samples (1 to 6 months postpartum) were collected manually in the morning between 7 and 10 am. Samples were stored in a sterile tube. Before infant feeding, samples were taken of one breast in the morning. Each mother provided two 20 ml human milk samples (for a total of 700 samples). Samples were stored in sterile tubes and were transfe...
Ferric reducing, antioxidant power (FRAP) assay
The FRAP assay method was as previously explained by Benzie and Strain; based on the reduction of a Fe3+, tripyridyltriazine complex to the blue-colored ferrous form in the presence of reductants (antioxidants) . Briefly, 10 μL of the test sample, standard (FeSO4) or blank (for each milk sample, a blank sample was used to remove milk turbidity) was mixed with 250 μL of FRAP reagent, freshly prepared (10 volumes of 300 mM acetate buffer, plus1 volume of 10 mM TPTZ solution in 40 mM HCl, pl...
A-diphenyl- B-Picrylhydrazyl (DPPH) assay
The antioxidant activity of human milk samples was assessed in terms of free radical scavenging activity, according to the Brand-Williams method with a slight modification . Briefly, 50 μl of each human milk and control sample were mixed with 950 μl of the DPPH solution in a test tube. After a 10-minute incubation at 37 °C, it was centrifuged at 3000 g for 3minutes. The absorbance of the reaction mixtures was measured at 517 nm by a spectrophotometric method, and a methanol solution of DP...
Thiobarbituric acid reactive substances (TBARs) assay
The final product of lipid peroxidation, malonyldialdehyde (MDA), was measured using the TBARs assay in human milk samples . Samples (100 μl) were mixed with 1 ml of TBARs reagent (7.5 g trichloroacetic acid, 187 mg TBA, and 6.25 ml of chloridric acid). The mixture was heated for 20 minutes in a boiling water bath. Then 1 ml of N-butanol was used to extract TBARs adducts and the solution was centrifuged for 10 minutes at 1500 g at 4 °C. The fluorescence spectrum of these samples was measu...
All analyses were conducted using the Statistical Package for the Social Sciences (SPSS) version 16. Statistical data was measured for normality by using the Kolmogorov-Smirnov test. Continuous and categorical indices are displayed by Mean ± SD and number (percent), respectively. The ANOVA test was applied to measure the significant difference in n...
Research has shown that storage of both human milk and artificial baby milk, or infant formula, leads to a loss of ascorbic acid (commonly called Vitamin C). As milk is removed from the bottle during feeding and replaced by ambient air, it is unknown if loss of ascorbic acid occurs during the course of a feeding.
Maternal dietary vitamin and/or mineral supplementation, particularly fat- soluble vitamins, vitamin B1, B2 and C might be reflected in the breast milk composition. No difference was found between mega dose and single dose administration of minerals. Keywords: Dietary supplements, Human milk, Vitamins, Minerals, Breast-milk composition.
2021年8月9日 · Maternal nutritional status and dietary intake mainly influence milk concentrations of vitamins (A, D, B12, thiamin, riboflavin, pyridoxine), iodine, selenium and fatty acids profiles, whereas the milk content of proteins, carbohydrates and other minerals remains unaltered, unless the mother is extremely undernourished or depleted in ...
2015年10月28日 · Regarding the impact of postpartum women’s medicine use on breastfeeding, a few studies suggest that women’s use of certain medicines (e.g. antiepileptics, propylthiouracil, antibiotics) during lactation can reduce initiation and/ or duration of breastfeeding.
Breast-feeding [19], probiotic supplementation [20], vitamin C, and zinc supplementation [21] have been shown as pos-sible protective factors against type 1 DM whereas early exposure to eggs, gluten [22, 23] and vegetables [24] might increase the risk.
