搜尋結果
Short laser pulse. Flight tube and drift region to measure the time-of-flight (TOF) detector. Accelerating pulse. MALDI-TOF ionization. N. 2 laser pulse (337 nm) [M+H]+. [ [M-H]- - ]- matrix ions. Protein/peptide/nucleotide/saccharide deposited on crystal surface. How a TOF instrument works. Source Accelerating pulse.
- 276KB
- 14
Peptide analysis. Purity - ESI-MS is a revelation. Integration of MS with LC and CE. Multidimensional LC of peptides. Tandem MS. Identifying peptide amino acid sequences. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Advantages of MALDI-TOF. More tolerant to common buffers than ESI.
type I interferons (IFns) are essential mediators of antiviral responses. these cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies. despite a fundamental role in health and disease, the direct q...
Contd.. Pseudomolecular ion: Ion originating from the analyte molecule by abstraction of a proton [M-H]- or addition of proton [M+H]+. Tandem mass spectrometry (Cooks, 1976): MS/MS (McLafferty, 1978), tandem in space or time. Precursor ion/parent ion: Ions undergoing fragmentation. Product ion/daughter ion: Ions resulting from parent/precursor ...
Peptide mass fingerprinting. This method has been developed because of the availability of predicted protein sequences from genome sequencing. Proteins do not have to have been previously sequenced - only that the open reading frame in the gene is known - the rest is a virtual exercise in the hands of statisticians, bioinformaticists and computers.
PowerPoint Presentation. Development of an MRM Method. Infusion Pump (Harvard Apparatus) •Delivers constant supply of sample. Mass Spectrometer (AB/Sciex) •Ionization Chamber. •Electromagnetic Lenses. •Triple Quadrupole. •Detector. Quadrupole: An analyzer that stabilizes The trajectory of an ion based on the ion’s Mass to charge ratio (m/z).
4 天前 · B.S., National Tsing Hua University, Physics Ph.D., Stanford University, Physics I was born and raised in Penghu (the Pescadores Islands), an archipelago of 64 islets in the Taiwan Strait. People there have the luxury to enjoy fresh wild-caught seafood and extraordinary scenery of basalt rock ...
