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2021年1月4日 · A systematic review found that maternal dietary intake, particularly fatty acids, and some micronutrients, such as fat-soluble vitamins, vitamin B1 and C, was associated with micronutrient content in breast milk [ 2 ]. Micronutrients and vitamins are also very important factors for the development and growth of infants.
- Mojtaba Keikha, Ramin Shayan-Moghadam, Maryam Bahreynian, Roya Kelishadi
- 2021
- Study Setting
- Demographic and Anthropometric Variables Evaluation
- Milk Sampling
- Oxidant-Antioxidant Status Measurement
- Statistical Analysis
This cross-sectional study was performed at Birjand University of Medical Sciences on a sample of 350 mothers who were recruited between January–February 2021 from 4 health centers in different regions of Birjand, South Khorasan, Iran. Inclusion criteria included; healthy women between the ages of 20–35 years, with an infant 1–6 months old. We did ...
A trained nurse evaluated information including mother age, systolic blood pressure (SBP), type of delivery, body mass index (BMI), economic status, parent death, parent divorce, father s’ and mother s’ educational attainment, infant age, infant sex and infant head circumference. Participants’ height and weight were measured and BMI was calculated ...
Human milk samples (1 to 6 months postpartum) were collected manually in the morning between 7 and 10 am. Samples were stored in a sterile tube. Before infant feeding, samples were taken of one breast in the morning. Each mother provided two 20 ml human milk samples (for a total of 700 samples). Samples were stored in sterile tubes and were transfe...
Ferric reducing, antioxidant power (FRAP) assay
The FRAP assay method was as previously explained by Benzie and Strain; based on the reduction of a Fe3+, tripyridyltriazine complex to the blue-colored ferrous form in the presence of reductants (antioxidants) . Briefly, 10 μL of the test sample, standard (FeSO4) or blank (for each milk sample, a blank sample was used to remove milk turbidity) was mixed with 250 μL of FRAP reagent, freshly prepared (10 volumes of 300 mM acetate buffer, plus1 volume of 10 mM TPTZ solution in 40 mM HCl, pl...
A-diphenyl- B-Picrylhydrazyl (DPPH) assay
The antioxidant activity of human milk samples was assessed in terms of free radical scavenging activity, according to the Brand-Williams method with a slight modification . Briefly, 50 μl of each human milk and control sample were mixed with 950 μl of the DPPH solution in a test tube. After a 10-minute incubation at 37 °C, it was centrifuged at 3000 g for 3minutes. The absorbance of the reaction mixtures was measured at 517 nm by a spectrophotometric method, and a methanol solution of DP...
Thiobarbituric acid reactive substances (TBARs) assay
The final product of lipid peroxidation, malonyldialdehyde (MDA), was measured using the TBARs assay in human milk samples . Samples (100 μl) were mixed with 1 ml of TBARs reagent (7.5 g trichloroacetic acid, 187 mg TBA, and 6.25 ml of chloridric acid). The mixture was heated for 20 minutes in a boiling water bath. Then 1 ml of N-butanol was used to extract TBARs adducts and the solution was centrifuged for 10 minutes at 1500 g at 4 °C. The fluorescence spectrum of these samples was measu...
All analyses were conducted using the Statistical Package for the Social Sciences (SPSS) version 16. Statistical data was measured for normality by using the Kolmogorov-Smirnov test. Continuous and categorical indices are displayed by Mean ± SD and number (percent), respectively. The ANOVA test was applied to measure the significant difference in n...
Abstract. Background: This study aims to systematically review the effects of maternal vitamin and/or mineral supplementation on the content of breast milk. Methods: We systematically searched electronic databases including Medline via PubMed, Scopus and ISI Web of Science till May 24, 2018.
as Vitamin C (ascorbic acid) [5]. The L-enantiomer of ascorbic acid, commonly known as vitamin C, is a nutrient whose deficiency causes the dis-ease scorbuticus (scurvy). This essential nutrient is critical to the health and development of infants as it is for the
2008年8月11日 · Research has shown that storage of both human milk and artificial baby milk, or infant formula, leads to a loss of ascorbic acid (commonly called Vitamin C). As milk is removed from the bottle during feeding and replaced by ambient air, it is unknown if loss of ascorbic acid occurs during the course of a feeding.
- Jimi Francis, Kristy Rogers, Paul Brewer, Darby Dickton, Ron Pardini
- 2008
2023年7月28日 · Additionally, vitamins C and E were shown to reduce reactive oxygen species and have protective effects against cadmium exposure [38,39,40]. Taken together, these findings may indicate the need to encourage women of childbearing age to consume the recommended amounts of vitamins on regular basis.
vitamin and mineral supplements), milk onset and vol-ume, perceived milk supply, knowledge, and self-ecacy. Only articles available in English, those with primary experimental research design, and studies which used a behavioral intervention type weretype.
